Chromatography

Through informative articles and tutorials, we aim to demystify the complexities of HPLC, method development, and troubleshooting. Our team are passionate about sharing their knowledge and experiences, ensuring that you gain a deep understanding of HPLC principles and their practical applications.

Interview Questions and Answers on Chromatography

Fourier Transform Infrared (FTIR) Troubleshooting of Common Problems

Troubleshooting HPLC Pressure Fluctuation Problems (Unstable Baseline)

Use of UV and Diode-Array (PDA) Detectors in (U)HPLC

Diagnostics Tips and Tricks for Retention Time in HPLC

GC Column Change Procedure

Causes and Remedies for Loss of Precision in HPLC

Tips and Advice for Preparing your HPLC Pump

Maintenance of UV detector for Recycling Preparative HPLC

Maintaining and Cleaning the HPLC Flow Cell

Troubleshooting HPLC Peak Shape

Troubleshooting HPLC Peak Retention Variation

Drifting of Retention Time

How to Calculate Signal to Noise ratio process in LabSolutions (Shimadzu)?

Chromatography Basic Principles: Sample Preparations and Related Methods

Handbook of Basic Gas Chromatography

Troubleshooting HPLC Ghost Peaks

Handbook of Temperature Programmed Gas Chromatography

Troubleshooting HPLC Column Backpressure

Troubleshooting Changes in Retention Time in HPLC

Troubleshooting HPLC Autosampler Contamination

Maintenance of the HPLC Column

What is Dead Volume and Dwell Volume in HPLC?

What is distortion for Peaks in the Chromatogram?

Prevention of Retention Time Drifts in HPLC Analysis

HPLC Sample Hold Time

Audit Trails in HPLC: Key Considerations

Questions and Answers for HPLC

Understanding of HPLC Audit Trails

Preventing and Solving Common Hardware Problems in HPLC

GC Troubleshooting Tips

Good Laboratory Practice for HPLC

Troubleshooting Problems with Chromatogram

Troubleshooting Problems with Injector in HPLC

How to Justify HPLC Audit Trail Extra injections?

What is Ghost Peaks?

Troubleshooting Problems with Retention Variation

Troubleshooting Guide – HPLC

How to Audit HPLC Audit Trail?

Troubleshooting Abnormal Pressure in HPLC

Column Cleaning, Regeneration and Storage of Silica Based Columns

Types of HPLC Detectors

Do’s & Don’ts Regarding Operation of Gas Chromatography

Calculation of System Suitability Parameters

Troubleshooting for Increased Pressure

Common Mistakes to Avoid When Using HPLC Mobile Phases

Retention Time Variability in HPLC

Diagnostics Tips and Tricks for System Pressure Problems in HPLC

What is the Difference between HPLC and UPLC?

What is the Difference between HPLC and HPTLC?

Which Solvent is Better for Chromatography: Methanol or Acetonitrile?

Troubleshooting Problems Detected by Smell, Sight or Sound in HPLC

Why Degassing of HPLC Mobile Phase is Necessary?

Troubleshooting Leaks in HPLC

General HPLC Column Care

Troubleshooting Problems with Ghost Peaks

Troubleshooting Problems with Peak Shape (Peak Tailing, Split Peaks, Peak Fronting)

Peak Purity and Peak Identification

Abnormal Peak Shapes

What is Peak Tailing?

What is LC-MS Analysis?

How to Develop HPLC Method?

Checklist for Preventive Maintenance of HPLC

How to Make HPLC Columns last Longer?

Chromatographic Calculations

What is the Shelf Life for HPLC Columns?

What is the Expiry Date for HPLC Mobile Phase?

What is HPLC Column Conditioning? How Long to Equilibrate?

Do's and Don'ts: HPLC Analysis

Maintenance of Flow Cell

Daily and Routine LC Maintenance

Causes and Remedy for HPLC Troubleshooting

How to Select Buffer in HPLC Method Development?

Troubleshooting for UV Detector of HPLC

Column Restoration Procedures

HPLC Column Care and Maintenance

What is the Difference between C8 and C18 columns in HPLC?

Requirements for Analytical Method Development and Validation for HPLC

Use and Benefits of HPLC Guard Column

Water Used for HPLC Analysis

Troubleshooting Problems with Column Backpressure

What is the RSD Limit for Replicate Standards in HPLC Analysis?